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Subpart C - Urine Collection Personnel 

§40.31   Who may collect urine specimens for DOT drug testing?      

(a) Collectors meeting the requirements of this subpart are the only persons authorized to collect urine specimens for DOT drug testing. 


(b) A collector must meet training requirements of §40.33. 


(c) As the immediate supervisor of an employee being tested, you may not act as the 

collector when that employee is tested, unless no other collector is available and you are 

permitted to do so under DOT agency drug and alcohol regulations. 

(d) You must not act as the collector for the employee being tested if you work for a HHS- 

certified laboratory (e.g., as a technician or accessioner) and could link the employee with a urine specimen, drug testing result, or laboratory report. 


§40.33   What training requirements must a collector meet? 

To be permitted to act as a collector in the DOT drug testing program, you must meet 

each of the requirements of this section: 


(a) Basic information.   You must be knowledgeable about this part, the current “DOT 

Urine Specimen Collection Procedures Guidelines” and DOT agency regulations 

applicable to the employers for whom you perform collections and you must keep current

on any changes to these materials. The DOT Urine Specimen Collection Procedures 

Guidelines document is available from ODAPC  (Department of Transportation, 400 7th St, S.W., Room 10403, Washington DC, 20590, 202-366-3784 or on the ODAPC web site 



(b) Qualification training. You must receive qualification training meeting the requirements of this paragraph.  Qualification training must provide instruction on the following subjects: 

(1) All steps necessary to complete a collection correctly and the proper completion and 

transmission of the CCF; 

(2) “Problem” collections (e.g., situations like “shy bladder” and attempts to tamper with a 


(3) Fatal flaws, correctable flaws, and how to correct problems in collections; and 

(4) The collector’s responsibility for maintaining the integrity of the collection process, 

ensuring the privacy of employees being tested, ensuring the security of the specimen

and avoiding conduct or statements that could be viewed as offensive or inappropriate; 


(c) Initial Proficiency Demonstration. Following your completion of qualification training 

under paragraph (b) of this section, you must demonstrate proficiency in collections under 

this part by completing five consecutive error-free mock collections. 


(1) The five mock collections must include two uneventful collection scenarios, one insufficient quantity of urine scenario, one temperature out of range scenario, and one 

scenario in which the employee refuses to sign the CCF and initial the specimen bottle 

tamper-evident seal. 


(2) Another person must monitor and evaluate your performance, in person or by a means that provides real-time observation and interaction between the instructor and trainee

and attest in writing that the mock collections are “error-free.”  This person must be a 

qualified collector who has demonstrated necessary knowledge, skills, and abilities by - 


(i) Regularly conducting DOT drug test collections for a period of at least a year;  


(ii) Conducting collector training under this part for a year; or     


(iii) Successfully completing a "train the trainer" course. 


(d) Schedule for qualification training and initial proficiency demonstration.  The following

is the schedule for qualification training and the initial proficiency demonstration you must meet: 

(1) If you became a collector before August 1, 2001, and you have already met the requirements of paragraphs (b) and (c) of this section, you do not have to meet them again. 

(2) If you became a collector before August 1, 2001, and have yet to meet the require-ments of paragraphs (b) and (c) of this section, you must do so no later than January 31, 2003.   

(3) If you become a collector on or after August 1, 2001, you must meet the requirements of paragraphs (b) and (c) of this section before you begin to perform collector functions. 


(e) Refresher training. No less frequently than every five years from the date on which yousatisfactorily complete the requirements of paragraphs (b) and (c) of this section, you must complete refresher training that meets all the requirements of paragraphs (b) and (c) of 

this section. 


(f) Error Correction Training.   If you make a mistake in the collection process that causes a test to be cancelled (i.e., a fatal or uncorrected flaw), you must undergo error correction training.  This training must occur within 30 days of the date you are notified of the error

that led to the need for retraining.   


(1) Error correction training must be provided and your proficiency documented in writing by a person who meets the requirements of paragraph (c)(2) of this section. 


(2) Error correction training is required to cover only the subject matter area(s) in which

the error that caused the test to be cancelled occurred. 


(3) As part of the error correction training, you must demonstrate your proficiency in the 

collection procedures of this part by completing three consecutive error-free mock

collections.   The mock collections must include one uneventful scenario and two

scenarios related to the area(s) in which your error(s) occurred.  The person providing the 

training must monitor and evaluate your performance and attest in writing that the mock 

collections were “error-free.” 


(g) Documentation.  You must maintain documentation showing that you currently meet allrequirements of this section.  You must provide this documentation on request to DOT agency representatives and to employers and C/TPAs who are using or negotiating to use yourservices. 

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